Tissue Dissociation Enzymes for Adipose Stromal Vascular Fraction Cell Isolation: A Review

With the increasing clinical translation of adipose stromal cell based technologies, it is important to thoroughly report on all aspects of these technologies. In this article, we review the commonly used enzymes for adipose tissue dissociation and stromal vascular fraction isolation. The enzymes reviewed include collagenase, trypsin, clostripain, and the neutral proteases dispase and thermolysin. Enzyme characteristics, the advantages of enzymatic isolation, and other relevant practical and regulatory issues are discussed. Tissue Dissociation Enzymes for Adipose Stromal Vascular Fraction Cell Isolation: A Review Ryan A. Lockhart2, Cloe S. Hakakian2 and Joel A. Aronowitz1-3* 1Cedars-Sinai Medical Center, Los Angeles, CA, USA 2University Stem Cell Center, Los Angeles, CA, USA 3USC Keck School of Medicine, Los Angeles, CA, USA *Corresponding author: Joel A. Aronowitz, University Stem Cell Center, 8635 W. 3rd St. Suite 1090W Los Angeles, CA, USA, Tel: +1-310-659-0705; E-mail: [email protected] Received November 03, 2015; Accepted December 25, 2015; Published December 28, 2015 Citation: Lockhart RA, Hakakian CS, Aronowitz JA (2015) Tissue Dissociation Enzymes for Adipose Stromal Vascular Fraction Cell Isolation: A Review. J Stem Cell Res Ther 5: 321. doi:10.4172/2157-7633.1000321 Copyright: © 2015 Lockhart RA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.